- Alexandria Gazette I (MS331), 1942/19961
- Beebe collection of Washington family papers, 1818/1861, bulk 1840/18502
- George Washington collection, 1654/179995
- Historic ephemera collection, 1796/19861
- Historic manuscript collection, 1601/193331
- Historic map collection, 1675/19716
- Historic newspapers collection, 1757/19574
- Historical Photograph Collection, 1850/20102,486
- John Augustine Washington III and Mount Vernon Ladies' Association (MVLA) collection, 1834/19571
- John Augustine Washington III and family papers, 1789/199411
- Lawrence Washington collection of Washington family papers, 1787/19321
- Loeb Photography Collection (MS363), 1888/19411
- Martha Washington collection, 1761/18024
- Mount Vernon Black and White Film Collection, 1933/20051,157
- Robert Waldsmith Collection of stereographs and cabinet cards, 1856/1981153