- Early Records of the Mount Vernon Ladies' Association, 1853/1951, bulk 1853/187512
- Financial Records of the Mount Vernon Ladies' Association, 1858/20151
- Harrison H. Dodge Papers, 1816/19369
- Historic manuscript collection, 1601/19338
- Historical Photograph Collection, 1850/201064
- John Augustine Washington III and family papers, 1789/19946
- Lily Laughton Papers, 1850/18915
- Maps of Mount Vernon and the surrounding areas, 1857/19925
- Measured Drawings and Blueprints of Mount Vernon, 1870/20165
- Mount Vernon Black and White Film Collection, 1933/200515
- Papers of the Mount Vernon Ladies' Association, 1858/201611
- Papers of the Superintendent and Resident Director, 1850/1996167
- Publications and Printed Material of the Mount Vernon Ladies' Association, 1854/20163
- Superintendent's Letter Books, Diaries, and Monthly Reports - collection of bound volumes, 1885/196787