- Early Records of the Mount Vernon Ladies' Association, 1853/1951, bulk 1853/18751
- Harrison H. Dodge Papers, 1816/19364
- Historic manuscript collection, 1601/19331
- Historical Photograph Collection, 1850/20105
- John Augustine Washington III and family papers, 1789/19941
- Lily Laughton Papers, 1850/18911
- Measured Drawings and Blueprints of Mount Vernon, 1870/20161
- Mount Vernon Black and White Film Collection, 1933/20051
- Papers of the Mount Vernon Ladies' Association, 1858/20162
- Papers of the Superintendent and Resident Director, 1850/19963
- Publications and Printed Material of the Mount Vernon Ladies' Association, 1854/20161
- Superintendent's Letter Books, Diaries, and Monthly Reports - collection of bound volumes, 1885/19671