- Financial Records of the Mount Vernon Ladies' Association, 1858/20151
- Historical Photograph Collection, 1850/20101
- John Augustine Washington III and family papers, 1789/19941
- Morley Jeffers Williams Collection, 1602/19671
- Mount Vernon Black and White Film Collection, 1933/20051
- Operation Overview Collection, 1953/19831
- Oral Histories of the Mount Vernon Ladies' Association, 1957/20151
- Papers of the Mount Vernon Ladies' Association, 1858/20161
- Papers of the Superintendent and Resident Director, 1850/19961
- Publications - History of Mount Vernon and George Washington, 1854/20121
- Publications and Printed Material of the Mount Vernon Ladies' Association, 1854/20161
- Superintendent's Letter Books, Diaries, and Monthly Reports - collection of bound volumes, 1885/19671