- Audio Visual Collection, 1928/20154
- Edith Sprouse Subject Files, 1960/19866
- Financial Records of the Mount Vernon Ladies' Association, 1858/201521
- Frances Abbott Lyon Papers, 1976/19833
- Geo. Gregory Smart collection of Washington and Mount Vernon ephemera, 1793/201711
- George Washington Memorial Parkway History Collection, 1900/20013
- Guest Books and Visitor Registers, 1850/20162
- Historic ephemera collection, 1796/19863
- Historic manuscript collection, 1601/19331
- Historical Photograph Collection, 1850/20103
- John Augustine Washington III and family papers, 1789/19942
- Land Records of the MVLA, 1858/20062
- Maps of Mount Vernon and the surrounding areas, 1857/19922
- Measured Drawings and Blueprints of Mount Vernon, 1870/20165
- Mount Vernon Black and White Film Collection, 1933/20059