- U.S. Army Corps of Engineers, Photographs of Operations in Pennsylvania, Ohio, New York, and the Pacific Northwest1
- U.S. Forest Service, George Washington National Forest, Dry River District records7
- U.S. Forest Service, George Washington National Forest, Lost River and Massanutten Working Circles Reports2
- UR's 175th Anniversary Collection1
- USS Iowa (BB-4) Navy Band Photograph Album3
- USS Vulcan (AR-5) Collection3
- Ukrainian National Association. Assembly No. 29 (Nanticoke, Pa.) Records1
- Underwood & Underwood World War I press photographs36
- Union Carbide Corporation, Hawk's Nest Project, Papers2
- Unitarian Universalist Church of Norfolk Records13
- United Confederate Veterans Collection8
- United Daughters of the Confederacy Williamsburg (Va.) records8
- United Daughters of the Confederacy, Dr. Harvy Black Chapter Records15
- United Daughters of the Confederacy, General Jenkins Chapter, Scrapbooks of Records1
- United Daughters of the Confederacy, Radford, VA Chapter Records3