- Austin-Twyman Papers, 1765/193932
- John L. Nau III Civil War History Collection, 1806/1988, bulk 1861/186515
- James Washington Singleton Papers, 1770/1975, bulk 1850/192012
- Wharton and Radford Families Papers, 1783/190612
- Early Records of the Mount Vernon Ladies' Association, 1853/1951, bulk 1853/187510
- Geo. Gregory Smart collection of Washington and Mount Vernon ephemera, 1793/201710
- J. Hoge Tyler Family Collection, 1802/195610
- Manuscripts - Geographic Names, Business Names and Subjects, 1725/19499
- Cazenove Family Papers (MS212), 1786/19708
- Robert Morton Hughes Papers, 1754/19508
- Ancella Bickley, Historian, Research Papers regarding African-Americans, 1775/2018, bulk 1970/20117
- Black, Kent, and Apperson Family Papers, 1779/19847
- Brown, Coalter, Tucker Papers (II), 1791/19207
- Dickson Family Papers, 1769/19247
- Blow Family Papers, 1613/1960, bulk 1770/18756