- Abraham Lincoln Collection, 1860/19651
- Ackerly Family Papers4
- Administration records collection, 1776/197611
- African and African American photograph collection, 1850/19451
- Alexander Lockhart Nelson papers, 1848/19021
- Alexander Sterrett Paxton Papers, 1858/1959, bulk 1861/18653
- Alexander, Fultz, and Willson Families Collection, 1770/19701
- Alfred Leyburn Papers, 1823/1899, bulk 1830/18391
- Almanac collection, 1850/19092
- American Civil War collection, 1860/18953
- Anderson Family papers, 1755/195824
- Ann Smith Academy Papers, 1807/19101
- Anne McClung Collection, 1844/19663
- Baer Family Papers, 1744/19291
- Bank of Rockbridge Currency and Lexington Lottery Ticket collection, 1803/18622