- Austin-Twyman Papers, 1765/19391
- Blow Family Papers, 1613/1960, bulk 1770/18751
- Borland Family Papers, 1771/19401
- Bushrod Washington family papers, 1662/18352
- Carter Family Papers, 1667/1862, bulk 1792/18619
- Emory L. Kemp Papers regarding Industrial History, 1735/20211
- Historic Maps of Europe, 1572/18451
- International Association for Identification Records, 1842/2012, bulk 1910/19591
- Jerdone Family papers, 1753/18902
- Jim Comstock, Newspaper Editor and Collector, Papers, 1650/2003, bulk 1850/19951
- John W. Warner papers, 1967/2009, bulk 1967/20081
- Lexington Arsenal and Virginia Militia records, 1702/18391
- Manuscripts - Geographic Names, Business Names and Subjects, 1725/19491
- Paul G. Caplan Papers, 1890/20041