- Philip S. Hench Walter Reed Yellow Fever collection, 1800/1998, bulk 1863/19745
- Bushrod Washington family papers, 1662/18352
- Historic Maps of Europe, 1572/18452
- Historic manuscript collection, 1601/19332
- Ann Norvell Otey Scott Papers, 1920/19481
- Anne H. Cutler Papers, 1930/2013, bulk 1954/20001
- Baer Family Papers, 1744/19291
- Betty Washington Lewis family papers, 1783/18441
- Bowman Family Papers, 1765/19981
- Chester McNerney Collection, 1290/19501
- Draft will of Elijah Forsythe, 1800/18201
- George Washington Custis Lee papers, 1875/19131
- Gordon M. Buck papers, 1881/1956, bulk 1910/19401
- Graham Family Papers, 1828/18941
- J. M. Shugar and A. W. Vaisey papers, 1832/19141