Elizabeth Brown Mulholland Gamble was born on May 8, 1900 in Charlottesville, Virginia. On October 19, 1925, she married Henry Bearden Mulholland, a professor of internal medicine at the University of Virginia from 1920-1966. Together they had two children, Elizabeth and Jack. Following Mulholland's death, Elizabeth married Edward W. Gamble Jr. in 1968. She died March 15, 2001 at the age of 101 in a nursing home in Maryland.

For over six decades, Elizabeth Brown Mulholland Gamble was exceedingly active in volunteer efforts for the University, the medical school, and hospital. She used her talents to improve UVa's clinical and educational facilities and enhance services to patients and their families. Her most significant accomplishment was to establish the Pink Lady Services Organization in 1951. By Gamble's own accounts, she raised over $90,000 for medical departments which included the purchases of the first cobalt machine, the first electron microscope, two specially equipped vans to test children for deafness in public schools throughout Virginia, and funds for landscaping of the Children's Rehabilitation Center. Gamble was also a passionate tennis player. She wrote her autobiography in 1974 and titled it "She Dares to Be Different."