The Norfolk & Western Railroad was organized in 1881 from the Atlantic, Mississippi, and Ohio Railroad. Primarily a line carrying agricultural products at its inception, the Norfolk and Western rapidly became associated with the mineral development of the southwestern part of Virginia and West Virginia. In 1881 it acquired the franchises to four other lines: the New River Railroad, the New River Railroad, Mining, and Manufacturing Company, the Bluestone Railroad, and the East River Railroad. These companies became the basis for Norfolk and Western's New River Division, which ran to the coalfields to the west.

Much of the early history of the Norfolk & Western Railway can be written in terms of expansion and consolidation with other lines. In 1890, it acquired the Shenandoah Valley Railroad, which ran from Roanoke, Virginia, to Hagerstown, Maryland. By 1891, an Ohio extension was well underway, giving the railroad access to the industrial Midwest. In 1892, Norfolk & Western Railroad leased the Roanoke and Southern Railroad, connecting Roanoke with Winston-Salem, North Carolina, and in 1893 it leased the Lynchburg and Durham, connecting Lynchburg with Durham, North Carolina. But this program of expansion, coupled with the economic depression of the 1890s, forced the railroad into receivership in 1895. It emerged as the reorganized Norfolk and Western Railway the next year.

Through expansion and various mergers, the Norfolk & Western Railway grew to become a major U.S. carrier, serving 14 states. The company manufactured steam locomotives at its own Roanoke Machine Works (established in Roanoke, Virginia in 1882), and among the railroad's affiliated holdings were several hotels (represented in this collection by the Bluefield Inn and the Hotel Roanoke). Among the company's larger merger acquisitions was the New York, Chicago and St. Louis Railroad--the "Nickel Plate Road"--in 1964. In 1982, the Norfolk & Western Railway combined with the Southern Railway to form Norfolk Southern Corporation.